Quick DNA Extraction of CRISPR KnockIn Cells Using Lucigen Quick DNA

dilutions of the QuickExtract extract after sample processing is advised, for example 1:4, 1:8, 1:16, to determine the optimal amount of processed sample volume for your downstream application. Finally, it is important to emphasise that seeds must be cleaned efficiently if genotyping, since interpretation of results may

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The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis. The result is easy processing of one to hundreds of samples in minutes, with no sample loss or toxic organic solvents.

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Apply for a Business Account Chemical Structure Search Favorites Help & Support Quick Order. Promotional price valid on web orders only. Your contract pricing may differ.. The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube.

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Simple, rapid extraction (no purification) of DNA for PCR-based screening and genotyping type applicationsThe wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. Use these reagents for isolating and purifying nucleic acids from biological material for use in further.

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QuickExtract DNA Extraction Solution. The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.

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The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes (Fig. 1), depending on the sample. QuickExtract Solution has been used to extract DNA from samples such as hair follicles, quill-end cells of.

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protocol has been used to extract PCR-ready genomic DNA from HeLa cells, hair follicles, tail snips, bacterial cells, fingernails, and feathers. 1. Label the appropriate number of tubes containing 0.5 ml of QuickExtract Solution. 2. Place one sample in each tube, for example: • 10. 4. counted human cervical carcinoma tissue culture (HeLa) cells.

Quick DNA Extraction of CRISPR KnockIn Cells Using Lucigen Quick DNA

Fast: 8 minute extraction protocol for most sample types. Simple: No centrifugation steps of spin columns used to help increase yields. Automation-friendly: Simple protocol integrated easily into automated workflows. Safe: Uses only non-toxic reagents. These products are suitable for a broad range of sample types, from swabs to hair follicles.

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QuickExtract DNA Extraction Solution. QuickExtract™ DNA Extraction Solution is a simple and rapid extraction method of PCR-ready DNA for screening and genotyping applications from hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells and other sample types. QuickExtract™ DNA Extraction Solution is a simple and.

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Ideal for DNA and RNA screening or genotyping, QuickExtract™ Extraction Kits make the process straightforward and efficient, enabling the rapid extraction of PCR-grade genomic DNA from any sample type. All of this is achieved in 3-8 minutes using a single-tube protocol, eliminating the need for cumbersome equipment like centrifuges or spin.

QuickExtract™ DNA Extraction Solution

Extract DNA from any sample in minutes with QuickExtract™ Solution. PCR-ready, high-quality genomic DNA for various applications.

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Mix by vortexing for 15 seconds. 2. www.lucigen.com QuickExtractTM DNA Extraction Solution. 4. Transfer the tube to a heat block at 65°C and incubate for 6 minutes (15 minutes for fingernails). 5. Mix by vortexing for 15 seconds. 6. Transfer the tube to a heat block at 98°C and incubate for 2 minutes.

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The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample. The extracted DNA is suitable for PCR analyses, such as genomic, transgenic, or viral DNA screening in animals, or.

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The extraction procedure consists of incubating the tissue section in 100 µl extraction solution for 1 h at 56°C, then for 2 min at 98°C. The genomic DNA in the resulting mixture is ready for PCR without further purification, or it can be stored at -20°C or -80°C. The extraction protocol can be used on FFPE sections that have been cut and.

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QuickExtract DNA Extraction Solution • The QuickExtract™ DNA Extraction Solution extracts PCR-ready genomic DNA from almost any sample in just 3-8

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Pellet the cells by centrifugation at 1,000 x g at 4°C for 5 minutes. Aspirate the supernatant. Add 100 μl of ice cold QuickExtract RNA Extraction Solution to the cells and vortex mix for 1 full minute. The RNA can be used directly in a cDNA synthesis reaction or stored frozen at -70°C.